International Journal of Innovative Research in Engineering and Management
Year: 2015, Volume: 2, Issue: 5
First page : ( 37) Last page : ( 42)
Online ISSN : 2350-0557.
Dr. Roberto A. Lima Dra. Arminda Saconi Dra. Galba M. Campos-Takaki , Dra. Rosileide F.S.Andrade Msc. Dayana R. Montero
CoQ10 is an endogenous antioxidant that is present in all tissues, varying in quantity and function, and is used to reduce oxidative stress, marketed as a dietary supplement and used in cosmetic formulations. This study aimed to investigate the production of CoQ10 by Candida glabrata using an optimized medium, compound of agro-industrial wastes (whey and corn steep liquor), evaluated the production, its cytotoxic effect and antioxidant potential. The production of CoQ10 was initially performed in synthetic medium and production medium optimized, with replacement of glucose by whey and peptone by corn steep liquor as substrates. Concentrations were established by experimental design (CCD) 22 . The optimized medium in the condition 4 (40% whey and 20% corn steep liquor) presented the higher biomass yield of 9.1g/L-1 and the CoQ10 production was confirmed after analysis comparative of the retention time of the standard with the sample, in terms of quantification CoQ10 obtained a value yield of 150μg/g. Determination of cytotoxic activity was performed by the method of cell viability in vitro using the dye MTT, in the concentration of CoQ10 25µg/mL, showed no cytotoxic activity on murine macrophages cells. The antioxidant potential was determined by the DPPH method, results of antioxidant activity showed a high antioxidant power of 73%. C. glabrata demonstrated to be an excellent producer of CoQ10 by biotechnological process with prospects for their use in industrial processes in the area of cosmetics.
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MSc.Valmir R. Cavalcante Dra. Galba M. Campos-Takaki Dra. Arminda S. Messias , MSc.Ricardo V. Trotski O. Silva Dr. Roberto A. Lima Dra. Kaoru Okada.
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